5BTB
Crystal Structure of Ashbya gossypii Rai1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.972, 164.239, 66.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.030 - 1.800 |
| R-factor | 0.18948 |
| Rwork | 0.187 |
| R-free | 0.22991 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.322 |
| Data scaling software | HKL-2000 |
| Phasing software | MR-Rosetta |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.355 |
| Number of reflections | 38099 | |
| <I/σ(I)> | 24 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M phosphate-citrate (pH4.2), 20%(w/v) PEG300, 0.2M ammonium sulfate, 10%(v/v) glycerol |
