5BSZ
X-ray structure of the sugar N-methyltransferase KedS8 from Streptoalloteichus sp ATCC 53650
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-02 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 63.254, 81.004, 126.916 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.710 - 2.000 |
R-factor | 0.17062 |
Rwork | 0.168 |
R-free | 0.22577 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bxo |
RMSD bond length | 0.012 |
RMSD bond angle | 1.950 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.045 | 0.143 |
Number of reflections | 21803 | |
<I/σ(I)> | 14.3 | 4.2 |
Completeness [%] | 97.6 | 94 |
Redundancy | 3.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 28-33% MPD, 2.5 mM SAH, 100 mM MOPS |