5BS7
Structure of histone H3/H4 in complex with Spt2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 121.205, 121.205, 118.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.200 - 3.300 |
| R-factor | 0.23282 |
| Rwork | 0.230 |
| R-free | 0.29025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.760 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.200 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.093 | |
| Number of reflections | 13876 | |
| <I/σ(I)> | 21.6 | 1.1 |
| Completeness [%] | 99.0 | 82.4 |
| Redundancy | 10.2 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.02 M NaCl, 0.2 M HEPES 7.5, 1.5 M ammonium sulfate |
