5BS5
EPSP synthase from Acinetobacter baumannii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-25 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.807, 103.896, 113.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.958 - 2.490 |
R-factor | 0.1749 |
Rwork | 0.172 |
R-free | 0.22490 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.791 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1682)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.958 | 50.000 | 2.570 |
High resolution limit [Å] | 2.490 | 6.780 | 2.490 |
Rmerge | 0.103 | 0.032 | 0.471 |
Total number of observations | 134827 | ||
Number of reflections | 30902 | ||
<I/σ(I)> | 12.6 | ||
Completeness [%] | 99.6 | 97.5 | 96 |
Redundancy | 4.3 | 3.9 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Bis-Tris Propane, pH 7.0, 0.1M Potassium Bromide, 40% PEG 8000 |