5BS1
Crystal structure of RbcX-IIa from Chlamydomonas reinhardtii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-06-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 |
Unit cell lengths | 36.133, 52.989, 61.561 |
Unit cell angles | 76.49, 81.10, 70.10 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.1787 |
Rwork | 0.177 |
R-free | 0.20600 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.509 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.870 | 48.870 | 1.580 |
High resolution limit [Å] | 1.498 | 4.730 | 1.498 |
Rmerge | 0.033 | 0.308 | |
Rmeas | 0.087 | ||
Rpim | 0.053 | 0.045 | 0.273 |
Total number of observations | 137620 | 5137 | 7139 |
Number of reflections | 56366 | ||
<I/σ(I)> | 10.7 | 24.2 | 2.4 |
Completeness [%] | 84.2 | 98.6 | 36.8 |
Redundancy | 2.4 | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 5 % PEG-3350, 0.2 M MgCl2, 50 mM Tris-HCl pH 8.0 |