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5BS1

Crystal structure of RbcX-IIa from Chlamydomonas reinhardtii

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyPIXEL
Collection date2010-06-25
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97900
Spacegroup nameP 1
Unit cell lengths36.133, 52.989, 61.561
Unit cell angles76.49, 81.10, 70.10
Refinement procedure
Resolution30.000 - 1.600
R-factor0.1787
Rwork0.177
R-free0.20600
Structure solution methodSAD
RMSD bond length0.011
RMSD bond angle1.509
Data reduction softwareXDS
Data scaling softwareSCALA (3.3.9)
Phasing softwareSHELX
Refinement softwareREFMAC (5.7.0029)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]48.87048.8701.580
High resolution limit [Å]1.4984.7301.498
Rmerge0.0330.308
Rmeas0.087
Rpim0.0530.0450.273
Total number of observations13762051377139
Number of reflections56366
<I/σ(I)>10.724.22.4
Completeness [%]84.298.636.8
Redundancy2.42.52
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82935 % PEG-3350, 0.2 M MgCl2, 50 mM Tris-HCl pH 8.0

227561

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