5BRJ
Structure of the bacteriophytochrome response regulator AtBRR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-18 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 41.046, 41.046, 187.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.896 - 1.922 |
R-factor | 0.2002 |
Rwork | 0.196 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RtBRR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.029 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.051 | 0.155 |
Number of reflections | 13163 | |
<I/σ(I)> | 53.2 | 23 |
Completeness [%] | 98.7 | 97 |
Redundancy | 27.2 | 26.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 200 mM magnesium chloride, 100 mM tris pH 8.5, 20% PEG 8000 |