5BR2
Structure of bacteriorhodopsin crystallized from ND-MSP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.973 |
Spacegroup name | P 63 |
Unit cell lengths | 61.030, 61.030, 110.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.600 - 1.800 |
R-factor | 0.19541 |
Rwork | 0.193 |
R-free | 0.23679 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.069 | 1.045 |
Number of reflections | 21165 | |
<I/σ(I)> | 20 | 2.26 |
Completeness [%] | 98.4 | 100 |
Redundancy | 8.9 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | The crystals were grown using the in meso crystallization method |