5BQZ

Crystal structure of hemagglutinin of A/Chicken/Guangdong/S1311/2010 (H6N6) in complex with human-like receptor LSTc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	80
Detector technology	CCD
Collection date	2014-10-02
Detector	RAYONIX MX-300
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	69.620, 138.630, 199.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.935 - 2.890
R-factor	0.2277
Rwork	0.225
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bny
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	47.950
High resolution limit [Å]	2.890
Number of reflections	39833
<I/σ(I)>	8.3
Completeness [%]	91.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1 M Bis-Tris propane and 2.3 M (NH4)2SO4