5BQY
Crystal structure of hemagglutinin of A/Chicken/Guangdong/S1311/2010 (H6N6) in complex with avian-like receptor LSTa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-10-24 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.350, 137.770, 199.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.873 - 2.780 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bny |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.937 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.130 |
| High resolution limit [Å] | 2.780 |
| Number of reflections | 46081 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 99.7 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.1 M Bis-Tris propane (pH 8.0) and 2.3 M (NH4)2SO4 |
