5BQX
Crystal structure of human STING in complex with 3'2'-cGAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2014-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.061, 111.061, 35.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.668 - 2.000 |
R-factor | 0.1908 |
Rwork | 0.187 |
R-free | 0.22630 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.039 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 15799 |
<I/σ(I)> | 27.6 |
Completeness [%] | 100.0 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.8, 25% PEG4000 |