5BQL
Fluorescent protein cyOFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.016230 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.124, 102.764, 123.254 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.390 |
R-factor | 0.1955 |
Rwork | 0.193 |
R-free | 0.25220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.826 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.000 | 2.530 | |
High resolution limit [Å] | 2.390 | 7.100 | 2.390 |
Rmerge | 0.084 | 0.036 | 1.022 |
Rmeas | 0.092 | 0.039 | 1.114 |
Total number of observations | 467689 | ||
Number of reflections | 38045 | 2759 | 11250 |
<I/σ(I)> | 14.8 | 39.69 | 1.94 |
Completeness [%] | 99.3 | 99.4 | 96.8 |
Redundancy | 6.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium chloride, 0.1M HEPES pH 7.5, 25% PEG 3350 |