5BQ9

Crystal structure of uncharacterized protein lpg1496 Legionella pneumophila subsp. pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	74.439, 76.625, 76.688
Unit cell angles	90.00, 96.66, 90.00

Refinement procedure

Resolution	32.363 - 2.279
R-factor	0.1822
Rwork	0.180
R-free	0.22630
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.594
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.278	6.240	2.278
Rmerge	0.095	0.036	0.861
Rmeas	0.103	0.039	0.936
Rpim	0.039	0.015	0.363
Total number of observations	265782
Number of reflections	38820
<I/σ(I)>	8.3
Completeness [%]	99.6	99	98.8
Redundancy	6.8	6.8	6.5
CC(1/2)		0.996	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	297	0.15M Magnesium Chloride, 0.1M Tris, 24% PEG 3350, 5% DMSO. 0.5M Calcium Chloride