5BPW
Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.987 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 139.181, 139.181, 156.106 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.943 - 3.400 |
R-factor | 0.2208 |
Rwork | 0.220 |
R-free | 0.27490 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.828 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.800 | 3.670 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.182 | 0.600 |
Number of reflections | 21671 | |
<I/σ(I)> | 5.16 | 2.3 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Apc4 crystals were grown by vapour-diffusion in a buffer containing 10 mM malic acid, 20 mM MES, 20 mM Tris.HCl (pH 8.0), 160 mM NDSB 196, 10 mM EDTA and 19% (v/v) PEG 1500. Crystals were incubated in a cryoprotection buffer comprising 10 mM malic acid, 20 mM MES, 20 mM Tris.HCl (pH 8.0), 160 mM NDSB 196, 10 mM EDTA, 22% (v/v) PEG 1500 and 20% (v/v) ethylene glycol prior to freezing in liquid nitrogen. |