5BPN
Crystal structure of nucleotide-free human Hsp70 NBD double mutant E268Q+R272K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.850, 83.348, 98.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.520 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3atv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.789 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP (11.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.520 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.189 | 0.753 |
| Number of reflections | 22618 | |
| <I/σ(I)> | 6.8 | 2 |
| Completeness [%] | 99.5 | 95.1 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25 % PEG-3350 W/V, 0.1 M HEPES |
