5BPL
Crystal structure of ADP and Pi bound human Hsp70 NBD mutant R272K.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.037, 63.327, 143.798 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.530 - 1.930 |
R-factor | 0.187 |
Rwork | 0.183 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3atv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.044 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP (11.0) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.530 | 1.970 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.141 | 0.843 |
Number of reflections | 31889 | |
<I/σ(I)> | 8.7 | |
Completeness [%] | 98.0 | 94.2 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25 % PEG-3350 W/V, 0.1 M HEPES |