5BPG
Crystal structure of the water-soluble FraC purified starting from the trans-membrane pore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.300, 44.440, 114.950 |
Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
Resolution | 47.190 - 2.140 |
R-factor | 0.21269 |
Rwork | 0.210 |
R-free | 0.26286 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vwi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.475 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | Aimless |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.190 | 2.210 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.099 | 0.310 |
Number of reflections | 42232 | |
<I/σ(I)> | 9.7 | 3.4 |
Completeness [%] | 97.5 | 89.3 |
Redundancy | 4.5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Microseeds generated in: 24% PEG 4000, 200 mM ammonium sulfate, 100 mM Cacodylate (pH 7.4) Single crystals generated in: 8% PEG 4000, 100 mM TRIS-HCl |