5BPD
Structure of TrmBL2, an archaeal chromatin protein, shows a novel mode of DNA binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.791, 58.670, 143.074 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 7.130 - 2.400 |
| R-factor | 0.2359 |
| Rwork | 0.234 |
| R-free | 0.28280 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.597 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 7.130 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.138 |
| Number of reflections | 62275 |
| <I/σ(I)> | 8.23 |
| Completeness [%] | 99.2 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Imidazole HCl, pH 8.0, 30% MPD, 10% PEG4000 |
