5BPB
Crystal structure of the cysteine-rich domain of human Frizzled 4 - Crystal Form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.608, 102.140, 116.508 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.200 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ijy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.279 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.770 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.077 | 0.911 |
Number of reflections | 44268 | |
<I/σ(I)> | 12.8 | 2 |
Completeness [%] | 99.2 | 99.7 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1.6 M tri-sodium citrate, pH 6.5 |