5BP8
ent-Copalyl diphosphate synthase from Streptomyces platensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 2 |
Unit cell lengths | 132.870, 132.870, 190.748 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.500 - 1.800 |
R-factor | 0.1579 |
Rwork | 0.156 |
R-free | 0.18840 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.420 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.077 | 0.053 | 0.635 |
Rmeas | 0.082 | 0.056 | 0.702 |
Rpim | 0.026 | 0.018 | 0.290 |
Total number of observations | 545528 | ||
Number of reflections | 59533 | ||
<I/σ(I)> | 9.5 | 2 | |
Completeness [%] | 99.4 | 99 | 93.3 |
Redundancy | 9.2 | 9.4 | 5.4 |
CC(1/2) | 0.998 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.5 M ammonium sulfate, 0.1 M Bis-Tris propane buffer, 5 mM magnesium chloride |