5BOX
Structure of TrmBL2, an archaeal chromatin protein, shows a novel mode of DNA binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.619, 105.790, 93.235 |
| Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
| Resolution | 7.410 - 2.500 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bpd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.410 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.150 | 3.064 |
| Number of reflections | 55688 | |
| <I/σ(I)> | 7.84 | 0.41 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 60% MPD, 0.1M HEPES pH 6.5 |
