5BO0
Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.2-H4 dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 112.964, 112.964, 98.226 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.915 - 2.906 |
R-factor | 0.1926 |
Rwork | 0.190 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.398 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 16336 | |
<I/σ(I)> | 25.7 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.3 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 3.2 M sodium formate, 0.1 M Tris, pH 8.5 |