5BNZ
Crystal structure of Glutamine-tRNA ligase /Glutaminyl-tRNA synthetase (GlnRS) from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-26 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 109.010, 56.020, 127.140 |
| Unit cell angles | 90.00, 99.41, 90.00 |
Refinement procedure
| Resolution | 41.809 - 1.900 |
| R-factor | 0.1883 |
| Rwork | 0.188 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nyl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_2027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.095 | 0.461 |
| Number of reflections | 119747 | |
| <I/σ(I)> | 11.71 | 3.31 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | Microlytics , MCSG1 C2 opt: 100mM BisTris pH 6.5, 200mM Lithium-sulphate, 23% PEG 3350; PsaeA.18222.a.B1.PW37623 at 20 mg/ml + 2.5mM AMPPNP and MgCl2; cryo: 20% EG + AMPPNP/Gln in two steps; tray 262504b8, puck bfw9-7 |
