5BNO

Crystal structure of human enterovirus D68 in complex with 6'SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9787
Spacegroup name	I 2 2 2
Unit cell lengths	325.700, 347.400, 356.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.260 - 2.150
Rwork	0.254
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mw8
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.152	0.068	0.865
Rmeas	0.178	0.087
Rpim	0.129	0.054	0.776
Total number of observations	1436142
Number of reflections	803405
<I/σ(I)>	5
Completeness [%]	74.6	67.1	75
Redundancy	1.8	2	1.6
CC(1/2)		0.985	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Sodium Acetate (pH 4.5), 3.5 M Sodium Formate