5BNH
Crystal structure of the HLTF HIRAN domain with a ssDNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.469, 50.117, 102.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.380 - 1.700 |
R-factor | 0.16719 |
Rwork | 0.165 |
R-free | 0.21353 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.933 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 51.380 |
High resolution limit [Å] | 1.700 |
Number of reflections | 28334 |
<I/σ(I)> | 3.9 |
Completeness [%] | 99.9 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 0.1 M Sodium acetate trihydrate pH 5.0, 40% 2-Methyl-2,4-pentanediol |