5BN7
Crystal structure of maltodextrin glucosidase from E.coli at 3.7 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 4 |
| Unit cell lengths | 110.590, 110.590, 69.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.610 - 3.700 |
| R-factor | 0.3304 |
| Rwork | 0.330 |
| R-free | 0.34402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.425 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.610 | 3.790 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.720 | |
| Number of reflections | 6156 | |
| <I/σ(I)> | 2.5 | 0.12 |
| Completeness [%] | 70.4 | 73.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.5 M ammonium acetate, 0.1 M sodium acetate |
