5BMP
Crystal Structure of Phosphoglucomutase from Xanthomonas citri complexed with glucose-1-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-10-23 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.861, 54.732, 173.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.563 - 1.850 |
R-factor | 0.1534 |
Rwork | 0.152 |
R-free | 0.17960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bmn |
RMSD bond length | 0.004 |
RMSD bond angle | 0.866 |
Data reduction software | XDS |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.960 | |
High resolution limit [Å] | 1.850 | 5.370 | 1.850 |
Rmerge | 0.020 | 0.017 | 0.041 |
Rmeas | 0.024 | 0.019 | 0.050 |
Total number of observations | 132372 | ||
Number of reflections | 36037 | 1591 | 5146 |
<I/σ(I)> | 45.06 | 62.88 | 22.89 |
Completeness [%] | 97.4 | 95.4 | 89.5 |
Redundancy | 3.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 12.5 % PEG 1000, 12.5 % PEG 3350, 12.5 % MPD, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazol pH 6.5 |