5BMP

Crystal Structure of Phosphoglucomutase from Xanthomonas citri complexed with glucose-1-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.861, 54.732, 173.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.563 - 1.850
R-factor	0.1534
Rwork	0.152
R-free	0.17960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bmn
RMSD bond length	0.004
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		1.960
High resolution limit [Å]	1.850	5.370	1.850
Rmerge	0.020	0.017	0.041
Rmeas	0.024	0.019	0.050
Total number of observations	132372
Number of reflections	36037	1591	5146
<I/σ(I)>	45.06	62.88	22.89
Completeness [%]	97.4	95.4	89.5
Redundancy	3.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	12.5 % PEG 1000, 12.5 % PEG 3350, 12.5 % MPD, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazol pH 6.5