5BKQ

Crystallographic structure of a cubic form of STMV grown from nitrate

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	173
Detector technology	CCD
Collection date	2002-06-19
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 2 3
Unit cell lengths	234.050, 234.050, 234.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.000 - 3.190
R-factor	0.2627
Rwork	0.261
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
RMSD bond length	0.003
RMSD bond angle	0.485
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.000	3.250
High resolution limit [Å]	3.180	3.180
Rmerge	0.143	0.235
Number of reflections	45796	6489
<I/σ(I)>	11.5	2.2
Completeness [%]	0.7
Redundancy	3.8
CC(1/2)	0.964	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	279	Crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. Drops were equal amounts of a 5 mg/ml virus stock solution buffered with 0.1 M phosphate at pH 6.5, and the reservoir solution which was 20% w/v sodium nitrate in 0.1 M phosphate at pH 6.0. Crystallization was carried out at 4 degrees C.