5BKL
Crystallographic structure of the cubic crystal form of STMV (77.9 degree rotation) grown from NaCl
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2002-06-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 3 |
Unit cell lengths | 234.650, 234.650, 234.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.220 - 2.940 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oq8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.715 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.500 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.170 | 0.320 |
Number of reflections | 87022 | 8458 |
<I/σ(I)> | 7.2 | 2.8 |
Completeness [%] | 96.0 | 75 |
Redundancy | 7.8 | 2.3 |
CC(1/2) | 0.970 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 279 | Crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. The drops were composed of equal amounts of a 5 mg/ml stock virus solution buffered at pH6.5 with 0.1 M phosphate, and the reservoir solution which was 8% w/v NaCl buffered with 0.1 M phosphate at pH 6.0. Crystallization was carried out at 4 degrees C. |