5BKH
The splicing activity and an alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-13 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9791 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 128.931, 128.931, 79.251 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.200 - 2.430 |
R-factor | 0.2426 |
Rwork | 0.239 |
R-free | 0.27440 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (v1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.310 |
High resolution limit [Å] | 2.270 | 6.160 | 2.270 |
Rmerge | 0.074 | 0.032 | |
Rmeas | 0.077 | 0.033 | |
Rpim | 0.023 | 0.009 | 0.435 |
Total number of observations | 227296 | ||
Number of reflections | 16787 | 896 | 839 |
<I/σ(I)> | 8.8 | ||
Completeness [%] | 91.0 | 85.1 | 93.5 |
Redundancy | 13.5 | 12.8 | 12.9 |
CC(1/2) | 1.000 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris HCl, pH 8.5, with 1.8 M ammonium sulfate, 0.1 M sodium chloride, and 1% glycerol |