5BK7
The structure of MppP E15A mutant soaked with the substrate L-arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.441, 200.514, 139.930 |
Unit cell angles | 90.00, 103.25, 90.00 |
Refinement procedure
Resolution | 45.401 - 2.196 |
R-factor | 0.2015 |
Rwork | 0.201 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dj1 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.308 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | 0.662 |
Number of reflections | 170041 | 7831 |
<I/σ(I)> | 14.9 | 1.7 |
Completeness [%] | 97.0 | 89.4 |
Redundancy | 5.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | 0.04 M citric acid, 0.06 M BIS-TRIS Propane, 20% PEG 3350 |