5BK7
The structure of MppP E15A mutant soaked with the substrate L-arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.441, 200.514, 139.930 |
| Unit cell angles | 90.00, 103.25, 90.00 |
Refinement procedure
| Resolution | 45.401 - 2.196 |
| R-factor | 0.2015 |
| Rwork | 0.201 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dj1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.662 |
| Number of reflections | 170041 | 7831 |
| <I/σ(I)> | 14.9 | 1.7 |
| Completeness [%] | 97.0 | 89.4 |
| Redundancy | 5.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | 0.04 M citric acid, 0.06 M BIS-TRIS Propane, 20% PEG 3350 |
