5BK5
Crystal structure of the anti-circumsporozoite protein 663 germline antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.177, 134.171, 111.796 |
| Unit cell angles | 90.00, 98.24, 90.00 |
Refinement procedure
| Resolution | 39.038 - 3.000 |
| R-factor | 0.2598 |
| Rwork | 0.257 |
| R-free | 0.31420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 663 Fab |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | SHELXPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.121 | 0.422 |
| Number of reflections | 36998 | 3460 |
| <I/σ(I)> | 6.41 | 1.6 |
| Completeness [%] | 98.4 | 98.6 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100 mM Tris pH 8.5, 200 mM MgCl2, 20% (w/v) PEG 4000 |
