5BK0
Crystal structure of 663 Fab bound to circumsporozoite protein NANP 5-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.815, 132.674, 51.020 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 38.297 - 3.150 |
| R-factor | 0.2365 |
| Rwork | 0.234 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 663 Fab |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | SHELXPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rpim | 0.102 | 0.347 |
| Number of reflections | 20694 | 1829 |
| <I/σ(I)> | 7.07 | 1.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 100 mM tri-sodium citrate pH 5.5, 20% (w/v) PEG 3000 |
