5BJR

Crystal structure of the N-terminal RRM domain from MEC-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	I 2 2 2
Unit cell lengths	52.590, 96.420, 109.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.360 - 2.600
R-factor	0.2421
Rwork	0.237
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3md1
RMSD bond length	0.002
RMSD bond angle	0.571
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.360	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.085	0.344
Number of reflections	8842
<I/σ(I)>	6
Completeness [%]	98.9
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M sodium acetate pH 6.5, 0.2 M ammonium sulfate, PEG 4000 30% w/v