5BCA
BETA-AMYLASE FROM BACILLUS CEREUS VAR. MYCOIDES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | WEISSENBERG IMAGE PLATE |
| Detector | PHOTON FACTORY |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.900, 112.900, 146.200 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| Rwork | 0.186 |
| R-free | 0.24000 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 25.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.300 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.208 |
| Total number of observations | 649891 * | |
| Number of reflections | 113923 | |
| <I/σ(I)> | 10.3 | 3.1 |
| Completeness [%] | 81.5 | 71.9 |
| Redundancy | 5.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 293 * | Oyama, T., (1998) Protein Pept.Lett., 5, 349. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium chloride | 50 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 6-8 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium chloride | 50 (mM) |
