5B7Z
Crystal Structure of Hyperthermophilic Thermotoga maritima L-Ketose-3-Epimerase with Ni2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97960 |
Spacegroup name | P 1 |
Unit cell lengths | 50.225, 55.220, 58.693 |
Unit cell angles | 107.16, 102.23, 92.35 |
Refinement procedure
Resolution | 41.513 - 1.500 |
R-factor | 0.1735 |
Rwork | 0.172 |
R-free | 0.19720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b7y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.112 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 90302 | |
<I/σ(I)> | 13.27 | 3.13 |
Completeness [%] | 96.0 | 92.8 |
Redundancy | 3.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 5%(w/w) PEG1000, 20%(v/v) PEG200 |