5B7Y
Crystal Structure of Hyperthermophilic Thermotoga maritima L-Ketose-3-Epimerase with Co2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.284, 55.255, 58.754 |
| Unit cell angles | 107.14, 102.35, 92.20 |
Refinement procedure
| Resolution | 35.152 - 1.320 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.18860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5JTX |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.340 |
| High resolution limit [Å] | 1.319 | 1.319 |
| Number of reflections | 130755 | |
| <I/σ(I)> | 20.75 | 5.9 |
| Completeness [%] | 94.7 | 90.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 5%(w/w) PEG1000, 20%(v/v) PEG200 |
