5B7U
Apo Structure of Cysteine Desulfurase from Thermococcus onnurineus NA1 at 1.89A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.488, 62.789, 171.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.177 - 1.900 |
| R-factor | 0.16751 |
| Rwork | 0.166 |
| R-free | 0.20309 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b7s |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.306 |
| Number of reflections | 66011 | |
| <I/σ(I)> | 54.02 | 9 |
| Completeness [%] | 97.0 | 91.9 |
| Redundancy | 9.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1mM PLP, 5mM cystine, 10% (v/v) Isopropanol, 5% (w/v) PEG 8000, Imidazol/HCl pH 5.9 |
