5B7P

Structures and functional analysis of periplasmic 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Aeromonas hydrophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.903
Spacegroup name	P 31 2 1
Unit cell lengths	102.725, 102.725, 118.653
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.157 - 1.490
R-factor	0.1351
Rwork	0.134
R-free	0.15550
RMSD bond length	0.009
RMSD bond angle	1.268
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.160
High resolution limit [Å]	1.490
Number of reflections	117632
<I/σ(I)>	37.59
Completeness [%]	99.6
Redundancy	16.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	288	6% (w/v) polyethylene glycol 4000, 0.1 M sodium acetate trihydrate (pH 4.6)