5B7N

Crystal structure of periplasmic 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Aeromonas hydrophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.985
Spacegroup name	P 31 2 1
Unit cell lengths	103.085, 103.085, 118.923
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.489 - 1.399
R-factor	0.1765
Rwork	0.175
R-free	0.19820
RMSD bond length	0.006
RMSD bond angle	1.066
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.490
High resolution limit [Å]	1.400
Number of reflections	142435
<I/σ(I)>	14.79
Completeness [%]	99.2
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	288	6% (w/v) polyethylene glycol 4000, 0.1 M sodium acetate trihydrate (pH 4.6)