5B7M

Structure of perdeuterated CueO - the signal peptide was truncated by HRV3C protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	49.911, 106.915, 262.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.485 - 1.800
R-factor	0.1667
Rwork	0.166
R-free	0.20410
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.121	0.884
Number of reflections	131300
<I/σ(I)>	15.1	1.98
Completeness [%]	99.7	98.1
Redundancy	6.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG 6000, 0.2 M lithium chloride, 0.1 M HEPES pD 7.0