5B7H
OxyR2 regulatory domain C206S mutant from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97960 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.703, 137.580, 96.914 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.808 - 1.492 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.19380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5B6U |
RMSD bond length | 0.015 |
RMSD bond angle | 1.339 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.099 | |
Number of reflections | 79030 | |
<I/σ(I)> | 10.9 | 7.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.9 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 287 | 0.2M ammonium sulfate, 0.1M sodium citrate (pH 5.6), 15% PEG 4000, 2mM TCEP |