5B7G
Structures and functional analysis of periplasmic 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Aeromonas hydrophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 102.671, 102.671, 118.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.639 - 1.399 |
R-factor | 0.1494 |
Rwork | 0.149 |
R-free | 0.16360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nm4 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.490 |
High resolution limit [Å] | 1.400 |
Number of reflections | 142443 |
<I/σ(I)> | 14.79 |
Completeness [%] | 99.2 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 288 | 6% (w/v) polyethylene glycol 4000, 0.1 M sodium acetate trihydrate (pH 4.6) |