5B7F
Structure of CueO - the signal peptide was truncated by HRV3C protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.513, 88.786, 53.951 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 35.110 - 1.450 |
| R-factor | 0.1532 |
| Rwork | 0.153 |
| R-free | 0.18100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.139 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.108 | 0.601 |
| Number of reflections | 82355 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.8 | 96.8 |
| Redundancy | 6.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20%(w/v) PEG6000, 0.2 M Calcium acetate, 0.1 M MES |
