5B78

Crystal structure of MOZ double PHD finger mutant-S210D/N235R in complex with histone H3 crotonylation at K14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	47.671, 48.238, 76.366
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.100 - 1.400
R-factor	0.1695
Rwork	0.169
R-free	0.18690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4llb
RMSD bond length	0.005
RMSD bond angle	0.787
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.100	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	35447
<I/σ(I)>	35.6
Completeness [%]	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	polyethylene glycol 4000, lithium sulfate, Tris