5B77

Crystal structrue of MOZ double PHD finger in complex with histone H3 propionylation at K14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	47.708, 48.028, 75.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.800 - 1.551
R-factor	0.1755
Rwork	0.174
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4llb
RMSD bond length	0.011
RMSD bond angle	1.257
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	25683
<I/σ(I)>	32.2
Completeness [%]	99.9
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	polyethylene glycol 4000, lithium sulfate, Tris