5B76

Crystal structure of MOZ double PHD finger domain in complex with histone H3 crotonylation at K14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97893
Spacegroup name	P 21 21 21
Unit cell lengths	48.120, 47.633, 76.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.060 - 1.653
R-factor	0.189
Rwork	0.187
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4llb
RMSD bond length	0.003
RMSD bond angle	0.628
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.100	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	21315
<I/σ(I)>	44.2
Completeness [%]	99.5
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	polyethylene glycol 4000, lithium sulfate, Tris