5B71

Crystal structure of complement C5 in complex with SKY59

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	CCD
Collection date	2015-02-05
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	39.790, 55.100, 127.760
Unit cell angles	89.18, 86.24, 78.20

Refinement procedure

Resolution	25.000 - 2.110
R-factor	0.2478
Rwork	0.246
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bbj 3cu7
RMSD bond length	0.007
RMSD bond angle	1.062
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.490	2.160
High resolution limit [Å]	2.110	2.110
Rmerge	0.072	0.317
Number of reflections	56154
<I/σ(I)>	7.9	1.8
Completeness [%]	92.1	95.8
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M magnesium formate dehydrate, 15.0% w/v polyethylene glycol 3350, 20% v/v glycerol as cryoprotectant