5B70

OxyR2 E204G regulatory domain from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 31 2 1
Unit cell lengths	141.375, 141.375, 109.664
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.957 - 2.300
R-factor	0.2293
Rwork	0.228
R-free	0.26750
RMSD bond length	0.003
RMSD bond angle	0.586
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.960	2.620
High resolution limit [Å]	2.300	2.300
Rmerge	0.089	0.368
Number of reflections	53831
<I/σ(I)>	9.67	2.46
Completeness [%]	95.3	88
Redundancy	5.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	287	0.2M sodium acetate, 0.1M Tris-HCl (pH 9.0), 14% PEG 4000, 2mM TCEP