5B6V
A three dimensional movie of structural changes in bacteriorhodopsin: resting state structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-02-04 |
| Detector | MPCCD |
| Wavelength(s) | 1.63 |
| Spacegroup name | P 63 |
| Unit cell lengths | 62.500, 62.500, 112.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.912 - 2.000 |
| R-factor | 0.1516 |
| Rwork | 0.150 |
| R-free | 0.17470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ns0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.732 |
| Data reduction software | CrystFEL (0.6.2) |
| Data scaling software | CrystFEL (0.6.2) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.920 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16786 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.1M Na/K phosphate pH5.4 , 30% PEG 2000 |
