5B6S
Catalytic domain of Coprinopsis cinerea GH62 alpha-L-arabinofuranosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.713, 77.735, 74.202 |
| Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
| Resolution | 74.100 - 1.700 |
| R-factor | 0.14062 |
| Rwork | 0.139 |
| R-free | 0.17017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b6t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.100 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.108 | 0.545 |
| Number of reflections | 59017 | |
| <I/σ(I)> | 15.1 | 2.7 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | MES buffer, NaBr, PEG 20000 |
